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Overland Park, KS – A groundbreaking study titled “Tailoring the Secretome Composition of Mesenchymal Stem Cells to Augment Specific Functions of Epidermal Regeneration: An In Vitro Diabetic Model,” conducted by, Jacob G. Hodge, Ph.D., introduces a pioneering strategy to revolutionize epidermal regeneration using the secretome of mesenchymal stem cells (MSCs) utilizing Ronawk’s Bio-Blocks™. Published in June 2023 in the journal
“Frontiers in Medical Technology” and available through open access, the study unveils a powerful solution for optimizing wound healing, particularly in a diabetic context.
Wound healing is an intricate process that is heavily reliant on paracrine factors to ensure proper wound healing progression. Inadequate epidermal regeneration, seen in chronic wounds like diabetic ulcers, can lead to prolonged healing timeframes and increased patient morbidity. Recent investigations into the dynamic secretome of Adipose-derived Mesenchymal Stem Cells (ASCs) have shown promise in enhancing the wound-healing response of chronic diabetic wounds. However, conventional 2D culture techniques negatively impact the regenerative phenotype of ASCs. To address this, the study introduces Ronawk’s novel tissue-mimetic 3D system, known as Bio-Blocks, which provides a more natural environment to culture ASCs.
Key highlights of the study:
● Power of a Tailored Secretome: The ASC secretome’s potential to augment epidermal regeneration activity was evaluated after ASC exposure to “wound priming stimuli” in both 2D and 3D environments.
● Diabetic Wound Care: To mimic diabetic wounds, keratinocytes (KCs) were exposed to elevated glucose levels, leading to a diabetic-like phenotype (idKCs).
● Enhanced Secretion with Bio-Blocks: ASC conditioned media (ASC-CM) from Ronawk’s tissue-mimetic culture exhibited over 50% increased protein secretion and a two-fold increase in secreted extracellular vesicles (EVs) compared to 2D culture.
● Precise Effect on Diabetic KCs: The study revealed significant differences in key epidermal regeneration factors within the ASC’s secretome, and the relative impact of ASC-EVs from Ronawk’s 3D tissue-mimetic system was particularly pronounced on the idKCs’ epidermal regeneration functionality.
Principal Investigator, A.J. Mellott Ph.D., states, “This research merges the cutting-edge potential of mesenchymal stem cell secretome with Ronawk’s Bio-Blocks to unlock new horizons in wound healing solutions. Our findings pave the way for innovative regenerative therapies in diabetic wound care as well as the standardization of Bio-Blocks as the culture platform of the future.”
The paper is available for download through open access at https://www.frontiersin.org/articles/10.3389/fmedt.2023.1194314/full, showcasing an extraordinary leap in wound healing strategies. Ronawk’s Bio-Blocks, the catalyst for this breakthrough, exemplifies innovation with implications for diabetic wound care and beyond.
About Ronawk
Ronawk’s Bio-Block Universe™ is the first expandable Bio-Factory designed to accelerate the development of biotechnology applications, processes, and technologies. By leveraging advanced mimetic culture technology, Ronawk’s Bio-Block Universe™ streamlines cell and tissue production, ultimately expediting research for next-generation therapies.
Ronawk’s Bio-Block Universe™ simplifies the once-tedious process of mimetic-culture workflows by minimizing labor, consumables, and space. Bio-Block™ technology employs biomimicry of soft tissues to optimize the growth of cells outside the body in a way that closely mirrors their natural growth within the body. This approach not only increases biological opportunities but also ensures cell viability, preservation of key characteristics, and secretion of therapeutic biologics. The process also decreases senescence and risks of contamination by eliminating subculturing from the process.
Ronawk’s Bio-Block™ platform is customizable, offering a consistent, repeatable, and scalable bio-mimetic microenvironment production that accelerates research and paves the way for innovative regenerative therapies. By harnessing the power of mimetic culture technology Ronawk is committed to transforming the field of biotechnology and advancing the development of life-changing treatments for patients in need.
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