The major challenge of iPSC-cultivation is to preserve their properties until differentiation is induced. To support this, an appropriate culture medium and a defined extracellular matrix-mimicking growth surface/coating are crucial. Biological coatings commonly used to expand stem cells are inherently complex, non-defined growth surfaces that frequently reduce experimental reproducibility.
However, in contrast to other types of stem cells, synthetic and ready-to-use growth surfaces are currently not available for human induced PSCs (hiPSCs). Eppendorf has now introduced a ready-to-use surface with synthetic fibronectin-derived motifs to support cell attachment by mimicking native ECM proteins.
According to the manufacturer, this novel surface supports long-term expansion of hiPSCs over 25 passages and is also suitable for hMSCs and other ECM-dependent cells. Furthermore, it allows expansion of stem cells in xeno-free and restrictive culture conditions, aiming to provide a completely defined culture system for PSCs without animal or human components.
Information about Eppendorf CCCadvanced FN 1 motifs cell cultureware, including detailed expansion analyses of hiPSCs and hMSCs, is available at www.eppendorf.com/ccc-